York Region Transit operates 104 daytime routes (68 Regular Routes, 3 Limited Express Routes, 10 GO Shuttles, 6 Express Routes, 34 School Specials and 5 Community Routes). 
There are 11 routes contracted to the Toronto Transit Commission and 2 Routes contracted to Brampton Transit.
| # | Route Name | Division | Route Type |   |   |   | Remarks | 
| 1 | Highway 7 |  | Regular | Richmond Hill Transit Centre | Box Grove Wal-Mart |  |  |  | 
| 2 | Milliken |  | Regular | GO Finch Bus Terminal | Denison St. & Markham Rd. |  |  | Route extended to Box Grove Wal-Mart on weekends and holidays
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| 3 | Thornhill |  | Regular | York University | Steeles Ave. E. & Don Mills Rd. |  |  |  | 
| 4 | Major Mackenzie |  | Regular | Vaughan Mills Terminal | Markland St. & Hillmount Rd. |  |  |  | 
| 4A | Major Mackenzie Dr. & Weston Rd. | Markland St. & Hillmount Rd. |  |  |  | 
| 5 | Clark |  | Regular | GO Finch Bus Terminal | Glen Shields Ave. & Dufferin St. |  |  |  | 
| 7 | Martin Grove |  | Regular | Woodbine Centre (Weekdays)
 Islington & Steeles Loop
 (Weekends)
 | Al Palladini Community Centre |  |  | No Sunday serviceSome weekday trips originate from Islington & Steeles Loop
 | 
| 8 | Kennedy |  | Regular | Kennedy Rd. & Steeles Ave. E. | Kennedy Rd. & Major Mackenzie Dr.
 |  |  |  | 
| 9 | 9th Line |  | Regular | Box Grove Plaza |  |  | Loop routeNo weekend service
 | 
| 10 | Woodbridge |  | Regular | York University (Weekdays)
 Weston Rd. & Highway 7
 (Weekends)
 | Martin Grove Rd. & Forest Dr. |  |  | Weekend service replaced with Dial-a-RideSome weekday trips operate via Kipling Ave. & Langstaff Rd.
 | 
| 12 | Pine Valley |  | Regular | Islington & Steeles Loop | Vaughan Mills Terminal |  |  | No Sunday serviceSome weekday trips operate via Four Valley Dr.
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| 13 | Islington |  | Regular | Islington & Steeles Loop | Napa Valley Ave. & Islington Ave. |  |  | No Sunday serviceSome weekday trips serve Kleinburg
 | 
| 14 | 14th Avenue |  | Regular | Denison St. & Woodbine Ave. | Box Grove Wal-Mart |  |  | No weekend serviceRush hour trips operate via Rouge Bank Dr. and Russell Jarvis Dr.
 | 
| 15 | Stouffville Road |  | Regular | Yonge St. & Jefferson Sdrd. | Millard St. & Automall Blvd. |  |  | Operates limited weekend service
 | 
| 16 | 16th Avenue |  | Regular | Rutherford Rd. & Ilan Ramon Blvd.
 | Markham Stouffville Hospital |  |  |  | 
| 17A | Birchmount |  | Rush Hour | Warden Subway Station | Crown Steel Dr. & Royal Crest Ct. |  |  | TTC-contracted routeNo weekend service
 | 
| 18 | Bur Oak |  | Regular | Angus Glen Community Centre | Markham Stouffville Hospital |  |  | No Sunday serviceSome weekday trips operate via Whites Hill Ave. and Cornell Rouge Blvd.Some weekday trips operate via Kenilworth Gt. and The Barley Lea St.
 | 
| 20 | Jane |  | Regular | York University | Mosque Gt. & Teston Rd. |  |  | Via Vaughan Mills TerminalWeekday service operates via Edgeley Blvd. & Millway Ave.Weekend service operates via Jane St.Some weekday trips operate via Creditstone Rd. & Locke St.
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